Journal: American Journal of Cancer Research

Article Title: REST-repressed lncRNA LINC01801 induces neuroendocrine differentiation in prostate cancer via transcriptional activation of autophagy

doi:

Figure Lengend Snippet: LINC01801 is a direct target of REST. (A) ChIP-seq data of REST binding sites in the LINC01801 promoter region (TSS ± 2000 bp) from the four cell lines PFSK-1, SK-N-SH, HepG2, and K562 were obtained from the ENCODE database. (B) ChIP-seq data of REST binding sites in the LINC01801 promoter region in LNCaP cells. (C) ChIP DNA from LNCaP-TR-shREST cells with or without Dox treatment was precipitated with anti-REST or anti-rabbit IgG and then amplified by qPCR using primers designed for the LINC01801 promoter. (D) RT-qPCR analysis of LINC01801 in LNCaP-TR-REST cells treated as indicated for 72 hours. For (C and D), data are presented as the mean ± SD. The statistical significance was calculated using Student’s t test. ***P<0.001.

Article Snippet: Antibodies were used in ChIP assays as follows: anti-REST (Millipore, 17-641) and nonimmune rabbit serum IgG (Alpha Diagnostic International).

Techniques: ChIP-sequencing, Binding Assay, Amplification, Quantitative RT-PCR